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multiplex pcr assay kit v 2  (TaKaRa)


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    Structured Review

    TaKaRa multiplex pcr assay kit v 2
    Multiplex Pcr Assay Kit V 2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pcr+assay+kit+v+2/Multiplex+PCR+Assay+Kit+Ver%2E2/pmc13107278-114-16-21
    Average 95 stars, based on 114 article reviews
    multiplex pcr assay kit v 2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Sex-Associated SNP Confirmation of Sex-Reversed Male Farmed Japanese Flounder Paralichthys olivaceus.
    Article Snippet: Female Japanese flounder Paralichthys olivaceus grow more rapidly than the male.. The goal of all-female commercial production requires an efficient method of genetic sex identification.. We conducted genome-wide association analysis of female and male farmed Japanese flounder (n = 24 per phenotypic sex) and found all regions of chromosome 24 to be significantly associated with phenotypic sex, suggesting it as the sex chromosome.

    Article Title: Phylogeography of a salmonid fish, white-spotted charr (<i>Salvelinus leucomaenis</i>), in a historically non-glaciated region in the northwestern North Pacific
    Article Snippet: 1Nikko Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Nikko, Tochigi 321-1661, Japan 2The University of Tokyo, Kashiwa, Chiba 277-8564, Japan 3Nagano Environmental Conservation Research Institute, Nagano, Nagano 381-0075, Japan 4Lake Biwa Museum, Kusatsu, Shiga 525-0001, Japan 5Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan 6Hokkaido University, Sapporo, Hokkaido 060-0811, Japan

    Article Title: Genome‐Wide Analysis Successfully Resolves Population Structure Shaped by Recent Divergence in the Endangered Bagrid Catfish Pseudobagrus ichikawai
    Article Snippet: MIG‐seq library preparation followed the original protocol (Suyama and Matsuki ), with some modifications, including changing the sequencing platform from MiSeq to NovaSeq (Illumina, USA) to enhance data volume and cost efficiency using modified primer sets (Onuki and Fuke ; Table ). .. The first round of PCR (1st PCR) used eight ISSR primer sets at 38°C, with the Multiplex PCR Assay Kit v.2 (Takara, Japan). ..

    Article Title: The genetic distinctiveness of Lethenteron satoi (Petromyzontidae) confirmed by nuclear SNP markers
    Article Snippet: The major modification was a change in sequencing platform from MiSeq to NovaSeq (Illumina, San Diego, CA, USA), using modified primer sets to improve both data yield and cost-efficiency (Onuki and Fuke 2022). .. The first round of PCR (1st PCR) was performed using eight ISSR primer sets with tail sequences at an annealing temperature of 38°C, with the Multiplex PCR Assay kit v.2 (Takara Inc., Shiga, Japan). ..

    Article Title: Skin mucus DNA with MIG-seq: a valuable combination for genome-wide SNP typing in aquaculture fish
    Article Snippet: Genome-wide SNP typing has garnered significant interest related to the growing demands for genetic research in farmed fish species.. This study validated skin mucus-derived DNA from red sea bream Pagrus major, a major aquaculture species in Japan as a viable source for the genotyping-by-sequencing technique MIG-seq.. Skin mucus samples were collected by swab from juvenile red sea bream, and DNA was subsequently extracted using Serapure magnet beads.

    Article Title: X-linked recessive ambicoloration with abnormal eye location in farmed Japanese flounder Paralichthys olivaceus
    Article Snippet: Preventing genetic disorders is essential to improving productivity and animal welfare in aquaculture.. We investigated ambicoloration with abnormal eye location (AEL), a unique morphological deformity frequently observed in farmed Japanese flounder Paralichthys olivaceus, often in specific broodstock groups, suggesting a genetic basis.. To identify the causative region, we conducted genome-wide association studies using MIG-seq as well as pool-seq techniques.

    Article Title: Clonal spore populations in sporocarps of arbuscular mycorrhizal fungi.
    Article Snippet: Some arbuscular mycorrhizal (AM) fungal species known to form sporocarps (i.e., aggregations of spores) are polyphyletic in two orders, Glomerales and Diversisporales.. Spore clusters (sporocarp-like structures) often formed in pot cultures or in vitro conditions are supposed to be clonal populations, while sporocarps in natural habitats with a fungal peridium are morphologically similar to those of epigeous sexual (zygosporic) sporocarps of Endogone species.. Thus, in this study, we explored the genetics of sporocarpic spores of two AM fungi with a view to possibilities of clonal or sexual reproduction during sporocarps formation.

    Article Title: Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.
    Article Snippet: The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan.. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood.. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds.

    Polymerase Chain Reaction:

    Article Title: Sex-Associated SNP Confirmation of Sex-Reversed Male Farmed Japanese Flounder Paralichthys olivaceus.
    Article Snippet: Female Japanese flounder Paralichthys olivaceus grow more rapidly than the male.. The goal of all-female commercial production requires an efficient method of genetic sex identification.. We conducted genome-wide association analysis of female and male farmed Japanese flounder (n = 24 per phenotypic sex) and found all regions of chromosome 24 to be significantly associated with phenotypic sex, suggesting it as the sex chromosome.

    Article Title: Phylogeography of a salmonid fish, white-spotted charr (<i>Salvelinus leucomaenis</i>), in a historically non-glaciated region in the northwestern North Pacific
    Article Snippet: 1Nikko Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, Nikko, Tochigi 321-1661, Japan 2The University of Tokyo, Kashiwa, Chiba 277-8564, Japan 3Nagano Environmental Conservation Research Institute, Nagano, Nagano 381-0075, Japan 4Lake Biwa Museum, Kusatsu, Shiga 525-0001, Japan 5Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan 6Hokkaido University, Sapporo, Hokkaido 060-0811, Japan

    Article Title: Genome‐Wide Analysis Successfully Resolves Population Structure Shaped by Recent Divergence in the Endangered Bagrid Catfish Pseudobagrus ichikawai
    Article Snippet: MIG‐seq library preparation followed the original protocol (Suyama and Matsuki ), with some modifications, including changing the sequencing platform from MiSeq to NovaSeq (Illumina, USA) to enhance data volume and cost efficiency using modified primer sets (Onuki and Fuke ; Table ). .. The first round of PCR (1st PCR) used eight ISSR primer sets at 38°C, with the Multiplex PCR Assay Kit v.2 (Takara, Japan). ..

    Article Title: The genetic distinctiveness of Lethenteron satoi (Petromyzontidae) confirmed by nuclear SNP markers
    Article Snippet: The major modification was a change in sequencing platform from MiSeq to NovaSeq (Illumina, San Diego, CA, USA), using modified primer sets to improve both data yield and cost-efficiency (Onuki and Fuke 2022). .. The first round of PCR (1st PCR) was performed using eight ISSR primer sets with tail sequences at an annealing temperature of 38°C, with the Multiplex PCR Assay kit v.2 (Takara Inc., Shiga, Japan). ..

    Article Title: Skin mucus DNA with MIG-seq: a valuable combination for genome-wide SNP typing in aquaculture fish
    Article Snippet: Genome-wide SNP typing has garnered significant interest related to the growing demands for genetic research in farmed fish species.. This study validated skin mucus-derived DNA from red sea bream Pagrus major, a major aquaculture species in Japan as a viable source for the genotyping-by-sequencing technique MIG-seq.. Skin mucus samples were collected by swab from juvenile red sea bream, and DNA was subsequently extracted using Serapure magnet beads.

    Article Title: X-linked recessive ambicoloration with abnormal eye location in farmed Japanese flounder Paralichthys olivaceus
    Article Snippet: Preventing genetic disorders is essential to improving productivity and animal welfare in aquaculture.. We investigated ambicoloration with abnormal eye location (AEL), a unique morphological deformity frequently observed in farmed Japanese flounder Paralichthys olivaceus, often in specific broodstock groups, suggesting a genetic basis.. To identify the causative region, we conducted genome-wide association studies using MIG-seq as well as pool-seq techniques.

    Article Title: Clonal spore populations in sporocarps of arbuscular mycorrhizal fungi.
    Article Snippet: Some arbuscular mycorrhizal (AM) fungal species known to form sporocarps (i.e., aggregations of spores) are polyphyletic in two orders, Glomerales and Diversisporales.. Spore clusters (sporocarp-like structures) often formed in pot cultures or in vitro conditions are supposed to be clonal populations, while sporocarps in natural habitats with a fungal peridium are morphologically similar to those of epigeous sexual (zygosporic) sporocarps of Endogone species.. Thus, in this study, we explored the genetics of sporocarpic spores of two AM fungi with a view to possibilities of clonal or sexual reproduction during sporocarps formation.

    Article Title: Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.
    Article Snippet: The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan.. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood.. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds.



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